Correlation between HPV PCNA, p16, and p21 Expression in Lung Cancer Patients

Purpose. Evaluate if human papillomavirus (HPV) infection in lung cancer patients might be helping development by altering p16, p21, and PCNA, key genes involved cell proliferation tumor development. Methods. 63 fresh-frozen (FF) formalin-fixed paraffin-embedded (FFPE) samples from were used to detect HPV PCR, followed genotype through sequencing. The host gene expressions of PCNA quantified qPCR both FF FFPE samples, the expression viral oncogenes E5, E6, E7 was also measured 19 samples. Results. 74.6% positive for HPV, 33/44 14/19 HPV-16 HPV-18 detected 31/33 7/33 FFPE, respectively, only type expressed 10/19, 2/19, 4/19 respectively. p16 RNAm higher HPV+ FFPE+FF while p21 showed all HPV- In turn, samples; however, a significant correlation as well E5 E7, inversely correlated p21. also, observed between HPV. FF+FFPE analysis, direct found HPV+, HPV-, an inverse HPV+. Also, protein 10 1 HPV-. Conclusions. Our data show that Northeast Brazil have high prevalence virus expresses its correlates with This could instigate studies focused on preventive strategies, such vaccination, prognostic indicator and/or individualized therapy.